Anthranilate Synthetase From Escherichia coli SJHI: Purification and Some Properties
نویسنده
چکیده
Abstract: A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially using concentrations greater than 10%. A positive effect 2-Mercapto-ethanol on the enzyme stability was also determined.
منابع مشابه
Anthranilate synthetase. Partial purification and some kinetic studies on the enzyme from Escherichia coli.
Anthranilate synthetase was purified by ammonium sulfate precipitation and gel filtration from extracts of an episomebearing Escherichia coli mutant grown under conditions of tryptophan pathway derepression. This purification represented an l&fold increase in specific activity over the activity of the derepressedmutant extract. Starch gel electrophoresis and ultracentrifugation revealed only sl...
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